3H System

Eliminating Biofilm in 3 Stages — Diagnosis, Removal and Long-Term Protection

Intelligent water sanitisation technology that protects production, reduces chemical usage, and supports the achievement of ESG objectives.

Why do traditional cleaning methods fail in the fight against biofilm?

A biofilm is a complex structure of microorganisms in which up to 90% forms a protective extracellular matrix.

Standard cleaning methods and chemicals only remove free-flowing bacteria in the system, leaving the source of contamination intact.

Secondary contamination Sudden contamination of the final product with bacteria.

Under-deposit corrosion Accelerated destruction of installations, especially stainless steel ones.

Flow resistance: Increased energy consumption and a drop in system performance.

Reputational risk Product recall and financial losses.

See how biofilm develops in installations

That’s why we developed the 3H System – an approach that eliminates biofilm at the source.

Why does the 3H System effectively eliminate biofilm where other methods fail?

Effective elimination of biofilm does not result from the application of a single chemical agent, but from a combination of appropriate diagnostics, technology, and process control.

System 3H combines these elements into a coherent, predictable solution tailored to the installation's operating conditions.

This is why System 3H is not a chemical – it's a complete technological solution for the permanent elimination of biofilm.

A comprehensive approach instead of a single measure: We don't act in a piecemeal fashion – we solve the problem at its source. The 3H system includes diagnosis, parameter selection, the biofilm removal process, and long-term system protection, ensuring the effect is not temporary.

The effectiveness of chlorine dioxide in biofilm structure: Chlorine dioxide (ClO₂) is one of the few substances capable of penetrating biofilm structures. This allows it to reach bacteria protected by the extracellular matrix and enables their effective removal – not just from the surface, but from the entire structure.

Precise fit for installation: Every installation requires a different approach.
The 3H system is custom-designed to suit the flow rates, materials, temperature and specific characteristics of the process, ensuring maximum operational efficiency.

Control and stabilisation of effects Biofilm elimination is a process, not a one-off action. The 3H System includes monitoring of effectiveness and maintenance of operating parameters, which prevents the regrowth of biofilm.

CUSTOMERS

The 3H System in 3 steps

From installation audits, biofilm removal, to long-term system protection. We tailor each stage to the specific needs of the plant and process requirements.

1

Audit and diagnosis

We are analysing the installation, identifying biofilm, and selecting a strategy for effective sanitisation.

installation analysis

microbiological studies

biofilm assessment

Risk analysis

Action plan

2

Cleaning and deployment

We remove biofilm, restore microbiological safety and implement a solution tailored to the installation.

biofilm removal

disinfection

Sanitization

parameter selection

system startup

3

Protection and stabilization

We monitor the effects, maintain microbiological stability and ensure long-term system protection.

monitoring

research

Calibration

parameter monitoring

maintaining the effect

RESULT

Laboratory-confirmed results

The graph shows the moment the 3H System was started. After 48 hours, the number of microorganisms in circulation drops below the detection limit, and microbiological stability is maintained at a constant level throughout the entire production cycle.

Microbiological examination of water in the drinking system

Comparison of results before and after the implementation of ProOxine® technology — pig farming, closed cycle

Before implementation: Downloaded 16.01.2026, result 25.01.2026 (Ref. 130/2026/B)

Upon deployment: Downloaded 04.02.2026, result 11.02.2026 (Ref. 285/2026/B)

Parameter

Before deployment

After the implementation of ProOxine®

Parameter

Coliform bacteria

jtk / 100 ml

Over 300

0

↓↓ elimination

Escherichia coli

jtk / 100 ml

21

0

↓↓ elimination

Faecal enterococci

jtk / 100 ml

49

0

↓↓ elimination

Coagulase-positive staphylococci

jtk / 100 ml

0

0

✓ no change

Total number of microorganisms at 22°C

jtk / 1 ml

Over 300

6

↓↓ >98%

Total number of micro-orgs. at 36°C

jtk / 1 ml

Over 300

2

↓↓ >99%

Salmonella spp.

Presence

Not found

Not found

Raw water samples taken from the drinking system before the ProOxine® dosing was activated and after the implementation period.

EHT Poland • ProOxine® Technology • Water hygiene in animal production

Microbiological parameters of water in the drinking system

Comparison of results before and after the implementation of ProOxine® technology — pig farming, fattening

Before implementation: Installation stand without ClO₂ sanitisation
Upon deployment: Constant ProOxine® dosing

Parameter

Before deployment

After the implementation of ProOxine®

Parameter

Coliform bacteria

jtk / 100 ml

200

0

↓↓ elimination

Escherichia coli

jtk / 100 ml

35

0

↓↓ elimination

Faecal enterococci

jtk / 100 ml

4

0

↓↓ elimination

Coagulase-positive staphylococci

jtk / 100 ml

0

0

✓ no change

Total number of microorganisms at 22°C

jtk / 1 ml

Over 300

Under 20

↓↓ >93%

Total number of micro-orgs. at 36°C

jtk / 1 ml

Over 300

Less than 10

↓↓ >96%

Salmonella spp.

Presence

Risk of presence

Not found

Elimination

Raw water samples taken from the drinking system before the ProOxine® dosing was activated and after the implementation period.

EHT Poland • ProOxine® Technology • Water hygiene in animal production

-30%

rinse water usage

-50%

spending on intervention chemistry

100%

reproducibility of microbiological test results

Efficiency in practice

Ongoing sanitisation vs. shock measures

Automatic activation systems

The effectiveness of chlorine dioxide does not depend solely on the quality of the preparation. The way it is activated and precisely dosed is also of key importance.

The System 3H uses automatic activation systems, developed by a team of experienced engineers, which eliminate the need for manual preparation of the working solution and guarantee user safety, process repeatability, and the maintenance of a stable ClO₂ concentration, ensuring effective microbiological control of the installation throughout its service life.

AANE™

Without mains power

An automatic activation system that automatically prepares and refills the tank with working solution exactly when it is needed.

 

Ideal for small and medium-sized installations requiring a simple and reliable solution.

EDIE™

Smart dosing

Electric activation system with a dual dosing system, utilising intelligent control for automatic preparation and precise dosing of chlorine dioxide solution.

It ensures high accuracy and stability of process parameters.

TITAN™

Maximum efficiency


An advanced PLC-controlled system, designed for large-scale installations with high chlorine dioxide requirements.



It enables the automatic preparation of a solution and maintains the set concentration even at very high flow rates.

From simple installations to the largest industrial plants - we select an activation and dosing system perfectly tailored to the needs of your facility.

AANE™

EDIE™

TITAN™

Small and medium-sized installations

Medium and large installations

Large-scale industrial installations

Without mains power

Smart dosing

PLC control

Automatic activation

Precise process control

Peak performance

Easy to use

Integration with automation systems

Work 24/7

At EHT, we do more than just supply products and equipment.

We design complete microbiological safety systems, combining specialist knowledge, proven technologies and intelligent automation.

CUSTOMERS

They placed their trust in us

Feedback

Customer testimonials

We have earned the trust of customers for whom hygiene, safety and process efficiency are of paramount importance.

ABOUT US

Over 15 years’ experience
in biosafety

15+

experience in international markets.

1000+

implementations carried out in industrial systems.

20

countries served
(Poland, Scandinavia, the Baltic States, Western Europe).

QUALIFICATIONS

Quality confirmed by tests and certificates

The effectiveness of our solutions is confirmed by research, approvals, and certificates recognised in demanding industries.

Certificates

OMRI Listed®

Organic Materials Review Institute

Health Certificate

National Institute of Public Health (PZH)
– State Research Institute

ISO 9001:2015

The Certification Body of TÜV SÜD America Inc.

Stay up to date

Knowledge base

Technological novelties, trade fair reports, and our latest achievements

FAQ

You most often ask

What is the 3H® system?

System 3H® is a comprehensive solution for maintaining the microbiological safety of water. It combines diagnostics, system sanitisation, active ClO₂ preparation, dosing, monitoring, and results reporting. The objective is to maintain a specific water quality standard, not merely to supply a chemical agent.

ProOxine is a preparation based on sodium chlorite, used to prepare active chlorine dioxide. It is one of the components of the 3H® system. Thanks to on-site activation, it is possible to obtain a fresh and effective ClO₂ solution.

The system prepares active ClO₂, doses it into the water installation, and supports the maintenance of appropriate microbiological parameters. The operation is based on continuous monitoring and regular effectiveness assessment. This allows the user to have control over water quality throughout the entire production cycle.

Active chlorine dioxide is produced in an activation device through the reaction of ProOxine with a citric acid-based activator. The process is carried out under controlled conditions, resulting in a solution with a predictable concentration. The solution is then dosed into the system.

Preparation on-site limits loss of activity and ensures high efficacy of the agent. Users do not need to store large quantities of the ready solution. The solution increases safety and improves process repeatability.

The Dosatron uses water flow to proportionally dose a substance. It does not require an electrical power supply and automatically adjusts the dosage to the current flow. This allows for a stable concentration of the substance to be maintained throughout the installation.

Yes. Chlorine dioxide is recognised as one of the most effective technologies for biofilm reduction. It can penetrate its structure and reduce sites for microbial growth.

Biofilm is a layer of microorganisms, organic matter, and metabolic products that deposits on installation surfaces. It forms a reservoir for bacteria and other pathogens. It can cause secondary contamination of water even when the quality of the water at the inlet is good.

Biofilm protects microorganisms from the action of many disinfectants. It hinders the maintenance of microbiological water quality and can limit the effectiveness of medicines administered through the system. It is one of the most common causes of chronic quality problems.

Yes. Laboratory studies and operational practice confirm high efficacy against many bacterial species relevant to animal production and water safety.

Yes. Efficacy against numerous viruses has been confirmed, including H5N1, adenoviruses, and noroviruses. This allows the technology to support biosecurity programmes.

Yes. In tests carried out in accordance with the PN-EN 17122 standard, a reduction of more than 6 log was achieved. This means that over 99.9999% of the tested virus population was eliminated.

This represents a very high level of disinfection efficacy. In practice, it means the removal of more than 99.9999% of the microorganisms present in the test sample.

Yes. Chlorine dioxide is commonly used to control Legionella. Its ability to act within biofilms and in extensive systems is particularly significant.

Yes, but selectively. It reacts primarily with specific chemical groups present in microorganisms and biofilm. This allows it to disinfect effectively without causing the classic chlorination characteristic of chlorine.

Yes. It oxidises selected amino acids and enzymes essential for the functioning of microorganisms. This is one of the main mechanisms of its biocidal action.

It can react with some fats, but much less so than ozone or free chlorine. This means that a larger proportion of the active agent can be used to combat microorganisms.

No classical resistance mechanisms characteristic of other disinfectants are observed. This is due to the different mechanism of action of ClO₂.

ORP is an indicator of the oxidation-reduction potential of water. It informs about the general chemical condition of the environment and can support the assessment of the effectiveness of a sanitisation process.

No. ORP is not a direct measure of chlorine dioxide concentration. It should be interpreted alongside measurements of active ClO₂ and other parameters.

Water is the most important nutrient for animals. Its quality affects the health of the digestive tract, feed intake and production results.

Yes. Microbiological problems can worsen feed utilisation and increase production costs. Therefore, water quality is one of the elements that affects the efficiency of farming.

A water quality management programme covering diagnostics, implementation, monitoring and reporting. Its aim is to maintain a defined microbiological safety standard.

Because the customer is buying the outcome of water quality and process control. Chemistry is just one part of the entire system.

It begins with a water audit and testing. Then, sanitation measures and monitoring are implemented. Effectiveness is confirmed by further testing.

New regulations require risk management across the entire water system. Requirements for monitoring, documentation, and control of microbiological hazards are increasing.

This is an investment in production stability. The costs of the programme are usually small compared to the potential losses resulting from health and production problems.

Yes. The analysis can include FCR, drug consumption, falls, and other economic parameters specific to the farm.

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